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Original Research: ASTHMA |

Adenosine Triphosphate Concentration of Exhaled Breath Condensate in Asthma

Zsófia Lázár, MD; László Cervenak, PhD; Márta Orosz, MD, PhD; Gabriella Gálffy, MD, PhD; Zsolt I. Komlósi, MD, PhD; András Bikov, MD; György Losonczy, MD, DMSc; Ildikó Horváth, MD, DMSc
Author and Funding Information

From the Department of Pulmonology (Drs Lázár, Orosz, Gálffy, Komlósi, Bikov, Losonczy, and Horváth), and the Research Group of Inflammation Biology and Immunogenomics (Dr Cervenak), Hungarian Academy of Sciences, Semmelweis University, Budapest, Hungary.

Correspondence to: Ildikó Horváth, MD, DMSc, Department of Pulmonology, Semmelweis University, Dios arok 1/C, Budapest, 1125, Hungary; e-mail: hildiko@elet2.sote.hu


For editorial comment see page 471

Funding/Support: This work was supported by the Hungarian Scientific Research Foundation [Grants 68808 and 68758].

Reproduction of this article is prohibited without written permission from the American College of Chest Physicians (http://www.chestpubs.org/site/misc/reprints.xhtml).


© 2010 American College of Chest Physicians


Chest. 2010;138(3):536-542. doi:10.1378/chest.10-0085
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Background:  Purinergic signaling is involved in asthma pathogenesis. Not only adenosine but also adenosine triphosphate (ATP) might play a role, but human evidence is scarce. ATP can be measured in exhaled breath condensate (EBC), a noninvasive airway sample suggested as being suitable for patient monitoring. We determined EBC ATP concentration in asthma, investigated its relation to disease parameters, and calculated airway ATP level.

Methods:  EBC was collected from 45 patients with persistent asthma (age 34.7 ± 13.2 years; FEV1, 87.0 ± 15.5% predicted; mean ± SD) and 32 healthy control subjects (age 36.9 ± 12.6 years; FEV1, 98.9 ± 9.9% predicted). Exhaled nitric oxide concentration (FeNO) and lung function were measured, and Asthma Control Test (ACT) score was obtained. EBC ATP was measured in luciferin-luciferase assay. Airway ATP concentration was calculated using dilution estimated from conductivity of vacuum-treated EBC samples. Parametric tests were applied in the analyses. ATP concentrations and nitric oxide levels were logarithmically transformed.

Results:  EBC ATP and calculated airway ATP concentrations were not elevated in asthma, and none of them was related to FeNO or ACT score. EBC ATP concentration was influenced by airway droplet dilution (r = −0.32, P < .05), and there was a relation between calculated airway ATP level and FEV1 (r = −0.35, P < .05).

Conclusions:  EBC ATP concentration does not seem to be useful for asthma monitoring. The relation between EBC mediator concentration and EBC conductivity highlights the importance of further standardization of EBC methodology and the need for more studies to understand airway droplet formation.

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