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Original Research: CRITICAL CARE MEDICINE |

Surfactant Protein-D and Surfactant Inhibit Endotoxin-Induced Pulmonary Inflammation*

Machiko Ikegami, MD, PhD; Elizabeth A. Scoville, BS; Shawn Grant, BS; Thomas Korfhagen, MD, PhD; William Brondyk, PhD; Ronald K. Scheule, PhD; Jeffrey A. Whitsett, MD
Author and Funding Information

*From the Division of Pulmonary Biology (Drs. Ikegami, Korfhagen, and Whitsett, Ms. Scoville, and Mr. Grant), Cincinnati Children’s Hospital, University of Cincinnati, Cincinnati, OH; and Genzyme Corporation (Drs. Brondyk and Scheule), Framingham, MA.

Correspondence to: Machiko Ikegami, MD, PhD, Professor of Pediatrics, Cincinnati Children’s Hospital, Division of Pulmonary Biology, 3333 Burnet Ave, Cincinnati, OH 45229-3039; e-mail: machiko.ikegami@cchmc.org



Chest. 2007;132(5):1447-1454. doi:10.1378/chest.07-0864
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Background: Acute lung injury is a common cause of morbidity and mortality following pulmonary or systemic infections. Surfactant protein-D is a member of the collectin family of proteins, which play important roles in innate host defense of the lung. In this study, the effect of exogenous recombinant human SP-D (rhSP-D) on protection of the adult mouse lung from lipopolysaccharide (LPS)-induced and lipoteichoic acid (LTA)-induced injury was assessed.

Methods: The effect of rhSP-D on LPS-induced and LTA-induced lung inflammation and injury was assessed with and without exogenous pulmonary surfactant in Sftpd+/+ and Sftpd−/− mice. A total of 204 mice (6 mice per group) were used for the present study.

Results: Sftpd−/− mice were more susceptible to intratracheal LPS than were Sftpd+/+ mice. rhSP-D decreased neutrophilic infiltrates induced by LPS and LTA in the lungs of both Sftpd+/+ and Sftpd−/− mice. The addition of exogenous pulmonary surfactant to rhSP-D further decreased LPS-induced and LTA-induced pulmonary inflammation in Sftpd−/− and Sftpd+/+ mice.

Conclusions: Intratracheal rhSP-D inhibited inflammation induced by intratracheal LPS and LTA instillation in the lung. The antiinflammatory effects of rhSP-D were enhanced by the addition of pulmonary surfactant, providing a potential therapy for the treatment of lung inflammation.

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