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Original Research: CYSTIC FIBROSIS |

Reproducibility of Nasal Potential Difference Measurements in Cystic Fibrosis*

Yasmin Yaakov, MSc; Eitan Kerem, MD; Yaakov Yahav, MD; Joseph Rivlin, MD; Hannah Blau, MD; Lea Bentur, MD; Micha Aviram, MD; Elie Picard, MD; Tali Bdolah-Abram, MSc; Michael Wilschanski, MBBS
Author and Funding Information

*From the Cystic Fibrosis Center (Ms. Yaakov, Dr. Kerem, Ms. Bdolah-Abram, and Dr. Wilschanski), Hadassah University Hospital, Jerusalem, Israel; Cystic Fibrosis Center (Dr. Yahav), Sheba Medical Center, Tel Hashomer, Israel; Cystic Fibrosis Center (Dr. Rivlin), Carmel Medical Center, Haifa, Israel; Graub Cystic Fibrosis Center (Dr. Blau), Schneider Children’s Hospital, Petach Tiqva, Israel; Cystic Fibrosis Center (Dr. Bentur), Rambam Medical Center, Haifa, Israel; Cystic Fibrosis Center (Dr. Aviram), Soroka Medical Center, Beer Sheva, Israel; and Cystic Fibrosis Center (Dr. Picard), Shaare Zedek Medical Center, Jerusalem, Israel.

Correspondence to: Michael Wilschanski, MBBS, Director Pediatric Gastroenterology Unit and Electrophysiology Laboratory, Hadassah University Hospital, Mount Scopus, Jerusalem, Israel 91240; e-mail: michaelwil@hadassah.org.il



Chest. 2007;132(4):1219-1226. doi:10.1378/chest.06-2975
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Background: Nasal potential difference (NPD) measurement has been advocated as a diagnostic tool for cystic fibrosis (CF) patients and as a method for assessing the response to new therapies. The purpose of this study was to examine the reproducibility of NPD measurements performed in a single center.

Methods: A total of 68 CF patients with a mean (± SD) age of 16 ± 8 years (age range, 6 to 52 years) underwent NPD measurements on at least two occasions.

Results: A total of 25 patients with classic CF (mean age, 21 ± 8 years) and 43 patients with nonclassic CF (mean age, 14 ± 8 years) underwent sweat tests and NPD measurements. The mean sweat chloride values were 102 ± 18 and 54 ± 14 mEq/L, respectively, for classic CF and nonclassic CF groups. All patients underwent repeat NPD measurements. The basal NPD and the response to amiloride (ΔAmil) and response to Cl free and isoproterenol (ΔCl free + iso) were very similar in both measurements. In the classic CF group, the basal potential difference values were −40 ± 12 vs −39 ± 11 mV (p = 0.57), respectively, for the first and second measurements; 27 ± 9 vs 26 ± 10 mV (p = 0.55), respectively, for ΔAmil; and 2.1 ± 3.8 vs 0.4 ± 2.9 mV (p = 0.07), respectively, for ΔCl free + iso. In the nonclassic CF group, the values were −32 ± 13 vs −28 ± 10 mV (p = 0.008), respectively; 19 ± 10 vs 17 ± 8 mV (p = 0.388), respectively; and −3.2 ± 4.6 vs −3.3 ± 4.4 mV (p = 0.876), respectively.

Conclusion: When performed in a single center, NPD is a reproducible test for CF patients and thus may be a useful outcome measurement for assessment of the efficacy of new treatments.

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