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Original Research: ASTHMA |

Proteomic Analysis of Peripheral T-Lymphocytes in Patients With Asthma*

Hye Cheol Jeong, MD, PhD; Sang Yeub Lee, MD, PhD; Eun Joo Lee, MD; Ki Hwan Jung, MD; Eun Hae Kang, MD; Sung Yong Lee, MD, PhD; Je Hyeong Kim, MD, PhD; Eun Kyung Park, PhD; Sang Hoon Lee; Chang Sub Uhm, MD, PhD; Yunjung Cho, MD, PhD; Chol Shin, MD, PhD, FCCP; Jae Jeong Shim, MD, PhD; Han Kyeom Kim, MD, PhD; Kwang Ho In, MD, PhD; Kyung Ho Kang, MD, PhD, FCCP; Se Hwa Yoo, MD, PhD
Author and Funding Information

*From the Division of Respiratory and Critical Care Medicine (Dr. Jeong), Department of Internal Medicine, College of Medicine, Pochon CHA University, Seongnam; Division of Respiratory and Critical Care Medicine (Drs. Sang Yeub Lee, E. J. Lee, Kang, In, and Yoo), Department of Internal Medicine, Anam Hospital, Korea University, Seoul; Division of Respiratory and Critical Care Medicine (Drs. Sung Yong Lee, Shim, and Kang), Department of Internal Medicine, Guro Hospital, Korea University, Seoul; Department of Internal Medicine (Drs. Jung, J. H. Kim, and Shin), Ansan Hospital, Korea University, Ansan; Department of Anatomy (Drs. Park, Uhm, and Mr. S. H. Lee), College of Medicine, Korea University, Seoul; Department of Laboratory Medicine (Dr. Cho), College of Medicine, Korea University, Seoul; and Department of Pathology (Dr. H. K. Kim), College of Medicine, Korea University, Seoul, Korea.

Correspondence to: Sang Yeub Lee, MD, PhD, Division of Respiratory and Critical Care Medicine; Department of Internal Medicine, College of Medicine, Korea University, 126–1, 5ga Anam Dong, Seongbuk gu, Seoul, 136–705, South Korea; e-mail: pulsy@korea.ac.kr



Chest. 2007;132(2):489-496. doi:10.1378/chest.06-2980
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Background: Asthma is chronic airway inflammation that occurs together with reversible airway obstruction. T-lymphocytes play an important role in the pathogenesis of asthma. Proteomic technology has rapidly developed in the postgenomic era, and it is now widely accepted as a complementary technology to genetic profiling. We investigated the changes of proteins in T-lymphocytes of asthma patients by using standard proteome technology: two-dimensional polyacrylamide gel electrophoresis (2D-PAGE), matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS), and a database search.

Methods: The proteins of CD3+ T-lymphocytes were isolated from whole blood of six steroid-naive asthmatic patients and of six healthy volunteers. 2D-PAGE was performed and the silver-stained protein spots were comparatively analyzed between the asthma and control groups using an image analyzer. Some differentially expressed spots were identified by MALDI-TOF-MS and database search. The messenger RNA expressions of some identified proteins were examined by real-time polymerase chain reaction (RT-PCR).

Results: Thirteen protein spots in the T-lymphocytes of the asthmatic patients were increased and 12 spots were decreased compared to those of the normal subjects. Among the identified proteins, the increased expression of the messenger RNA of phosphodiesterase 4C and thioredoxin-2 and the decreased expression of the messenger RNA of glutathione S-transferase M3 were confirmed by RT-PCR in the asthmatic patients.

Conclusions: Proteomic examination of the peripheral T-lymphocytes revealed some differentially expressed proteins in the asthmatic patients. The possibility of using the differentially expressed proteins as important biomarkers and therapeutic targets in asthma patients warrants further studies.

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