Secondary pulmonary hypertension (PH), frequently seen in patients with idiopathic pulmonary fibrosis (IPF), is associated with increased morbidity and mortality. The nature of the changes in vascular phenotypes that occur in IPF is still under debate. We intend to identify the transcription networks that underlie the abnormal vascular phenotype in IPF patients with secondary PH.
The database of the Simmons Center for Interstitial Lung Disease was queried to evaluate for the presence of PH by echocardiography or right-heart catheterization in IPF patients. Microarray analysis was performed on 13 of these IPF samples. Briefly, total RNA was extracted from surgical remnants of video-assisted thoracoscopic lung biopsy specimens and from lung explants from patients with IPF undergoing lung transplantation. Labeled complementary RNA was hybridized to oligonucleotide microarrays. Data analysis was performed using Scoregene and GeneExpress analysis packages.