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Clinical Investigations: ASTHMA |

Phenol-Containing Saline Solution as a Diluent for Adenosine 5′-Monophosphate in Bronchial Challenge Testing*

Luis Prieto, PhD; Carlos Badiola, MD; Julio Cortijo, PhD; Carmen Pérez-Francés, MD; Valentina Gutiérrez, PhD; Amparo Lanuza, PhD
Author and Funding Information

*From the Sección de Alergología, Hospital Universitario Dr Peset (Drs. Prieto, Pérez-Francés, Gutiérrez, and Lanuza), Valencia, Spain; Fundación de Investigación del Hospital General Universitario (Dr. Cortijo), Valencia; and GlaxoSmithKline (Dr. Badiola), Madrid, Spain.

Correspondence to: Luis Prieto, PhD, Sección de Alergología, Hospital Universitario Dr Peset, C/ Gaspar Aguilar 90, 46017 Valencia, Spain; e-mail: prieto_jes@gva.es



Chest. 2005;127(1):125-130. doi:10.1378/chest.127.1.125
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Objectives: To investigate the effect of dissolving adenosine 5′-monophosphate (AMP) with phenol-containing saline solution on the stability and the bronchoconstrictive properties of this indirect agonist.

Methods: Eleven subjects with asthma well controlled with short-acting inhaled β2-agonists as required or with inhaled corticosteroids were studied. Bronchial challenge tests with AMP dissolved with either normal saline solution or saline solution containing 0.4% phenol were performed on separate days. Furthermore, to assess the potential influence of the phenol-containing saline solution on the stability of the bronchoconstrictor agent, AMP solutions in concentrations of 40 μg/mL and 400 μg/mL were prepared in saline solution and phenol-containing saline solution and, after 30 min, the AMP levels were determined by high-performance liquid chromatography (HPLC) assay.

Results: The geometric mean AMP provocative concentration causing a 20% fall in FEV1 (PC20) was 13.49 mg/mL (95% confidence interval [CI], 6.76 to 26.91) for the saline solution method, and AMP PC20 for the saline solution with phenol method was 8.91 mg/mL (95% CI, 3.39 to 23.44) [p = 0.18]. No significant differences were found between the concentrations of AMP made in saline solution compared to those made in phenol-containing saline solution measured by HPLC.

Conclusion: These observations indicate that normal saline solution with or without phenol can be used as the diluent for AMP. However, since a potential risk with AMP of industrial sources is the bacterial contamination, adding a preservative such as phenol to a saline solution diluent might be recommended.

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