For buccal cell analysis, we adapted known techniques of RNA preservation and extraction. We have employed our novel RNA-specific reverse transcription polymerase chain reaction strategy that is unconfounded by genomic DNA pseudogene sequence encoding target and reference “housekeeper” sequences. All primers were blasted against bacterial sequences to assure human cell specificity of the amplification. Precise real-time quantitation of the expression of these genes employs ratios of target crossover point to reference housekeeper CRO (LightCycler; Roche; Basel, Switzerland). Genes that were analyzed included the aromatic hydrocarbon receptor gene (Ahr), cytochrome P450 genes CYP1A1 and CYP1B1, glutathione-S-transferase genes GSTM1, GSTM3, GSTP1, and GSTT1, nicotinamide adenine dinucleotide phosphate-quinone oxidoreductase (NQO1), copper-zinc superoxide dismutase (CuZn-SOD), catalase (CAT), and glutathione peroxidase (GPx). For any given individual among our initial group of human subjects, we have been able to reliably quantitate gene expression in the buccal cytologic specimens. We have compared buccal gene expression to laser capture microdissected lung gene expression, both by identical techniques of real-time quantitative reverse transcription polymerase chain reaction.