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Construction and Optimization of Chromosome Arm-Specific Comparative Genomic Hybridization Arrays for Identifying Genetic Alterations in Preinvasive Lung Cancers*

Cathie Garnis, BSc; Bradley Coe, BSc; Laura-Jane Henderson, BSc; Adrian Ishkanian, MSc; Spencer Watson, BSc; Marco Marra, PhD; John Minna, MD; Stephen Lam, MD, FCCP; Calum MacAulay, PhD; Wan Lam, PhD
Author and Funding Information

*From the British Columbia Cancer Research Centre (Mss. Garnis and Henderson, Mssrs. Coe, Ishkanian, and Watson, and Drs. Marra, S. Lam, MacAulay, and W. Lam), Vancouver, BC, Canada; and the Hamon Center for Therapeutic Oncology Research (Dr. Minna), University of Texas Southwestern Medical Center, Dallas, TX.

Correspondence to: Wan Lam, PhD, Senior Scientist, Cancer Genetics and Developmental Biology, British Columbia Cancer Research Centre, British Columbia Cancer Agency, 601 W 10th Ave, Vancouver, BC, Canada V5Z 1L3; e-mail: wanlam@bccancer.bc.ca



Chest. 2004;125(5_suppl):104S-105S. doi:10.1378/chest.125.5_suppl.104S
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The accumulation of genetic alterations is known to parallel lung cancer development. Array-based comparative genomic hybridization (aCGH) offers the potential for screening DNA samples for the deletion and amplification of chromosomal regions at a resolution 10 to 50 times greater than that of conventional comparative genomic hybridization (CGH) methodologies.

The construction and use of high-resolution CGH arrays will facilitate the fine mapping of known regions and the discovery of localized genetic alterations in lung cancer. Our objectives were as follows: (1) to construct high-density bacterial artificial chromosome (BAC) CGH arrays spanning selected chromosome arms annotated with genetic markers, which will anchor our data to publicly available loss of heterozygosity studies; (2) to optimize aCGH for the limited quality and quantity of microdissected DNA; (3) and to determine the impact of cell heterogeneity on aCGH.

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