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Genetic Predisposition to Lung Cancer* FREE TO VIEW

Ann G. Schwartz, PhD, MPH
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*From the Karmanos Cancer Institute, Detroit, MI.

Correspondence to: Ann G. Schwartz, PhD, MPH, Karmanos Cancer Institute at Wayne State University, 110 E Warren Ave, Detroit, MI 48103; e-mail: schwarta@med.wayne.edu



Chest. 2004;125(5_suppl):86S-89S. doi:10.1378/chest.125.5_suppl.86S
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In 2003, an estimated 171,900 new diagnoses and 157,200 deaths from lung cancer occurred in the United States.1While 80 to 90% of lung cancer incidence can be attributed to cigarette smoking, only 10 to 15% of all smokers develop lung cancer,2 and 10 to 15% of all lung cancers occur among nonsmokers. These figures suggest that there are individual differences in susceptibility to lung carcinogens and that these individual differences in susceptibility may be the result of genetic predisposition to lung cancer.

The hallmarks of an inherited disease include familial aggregation above that related to shared environmental or cultural exposures. Once familial aggregation is established, the pattern of inheritance of genetic susceptibility can be evaluated using segregation analysis to determine whether there is statistical evidence for the inheritance of a Mendelian major gene. If there is evidence supporting an inherited component to disease, the localization of disease genes is pursued.

The most definitive studies of the familial risk of lung cancer have included the collection of smoking and other risk factor information for all first-degree relatives. Four more recent studies36 of familial aggregation of lung cancer have been performed using a detailed family study approach, collecting risk factor data for family members. In a Louisiana family study,3after adjusting for age, sex, smoking history, and occupational exposures for each relative, a 2.4-fold excess of lung cancer was reported among relatives of lung cancer patients compared to relatives of spouse control subjects. In Detroit, a study was designed to focus on individuals who were likely to have a risk of lung cancer that was most strongly associated with genetic factors (ie, early-onset cases diagnosed < 49 years of age) and individuals who demonstrated increased susceptibility to low levels of exposure4 (ie, nonsmoking and early-onset cases). An excess risk of lung cancer, after adjustment for each relative’s age, sex, race, smoking status, occupation, industry, and history of other lung diseases, was seen in first-degree relatives of nonsmoking lung cancer cases diagnosed 40 to 59 years of age (relative risk, 6.1; 95% confidence interval [CI], 1.1 to 33.4),4and relatives of patients with early-onset cases (relative risk, 2.5; 95% CI, 1.7 to 3.6) [unpublished preliminary findings]. Familial risk was greater in African Americans (odds ratio [OR], 4.5; 95% CI 2.2–9.1) than in whites (OR, 1.8; 95% CI, 1.2 to 2.9). Mayne et al5also studied familial risk in relatives of nonsmoking men and women, and found elevated lung cancer risk in fathers, however, this finding was not significant (OR, 1.85; 95% CI, 0.80 to 4.33). A similar increased risk was reported from a case-control family study6 in Germany that was not limited to nonsmokers (OR, 1.67; 95% CI, 1.11 to 2.52).

These findings suggest a role for a genetic predisposition to lung cancer after taking into account the familial clustering of smoking habits, family size, and age structure. The findings of stronger aggregation when the onset of disease is early are indicative of an inherited component to risk.

Studies of familial aggregation do not explicitly reveal possible modes of inheritance underlying familial aggregation. Only two studies78 have investigated whether there is statistical evidence for the inheritance of a major gene for lung cancer using segregation analysis. Both studies78 have reported that the pattern of lung cancer occurrence in families is consistent with mendelian codominant inheritance of a rare autosomal gene. Sellers et al7 estimated that this putative gene is responsible for 69% of the lung cancer seen at age 50 years, 47% at age 60 years, and 22% at age 70 years. Yang et al9 reported that an environmental model with homogeneous risk across generations best explained the observed data in families of nonsmokers. However, among families of nonsmoking patients under the age of 60 years, a mendelian codominant model, with significant modifying effects of smoking and chronic bronchitis, best explained the observed data, with an estimated risk allele frequency of 0.004.8 Homozygous individuals with the risk allele are rare in the study population (1.6 per 100,000 population), making the attributable risk very low, even in the face of the very high penetrance of early-onset lung cancer (men, 85% by age 60 years; and women, 74% by age 60 years). One percent of the study population was made up of heterozygous individuals who had a relatively low risk of lung cancer, unless they were smokers with chronic bronchitis.

Findings of familial aggregation and statistical evidence for a major gene have led to the search for high-penetrant, rare, single genes for lung cancer and low-penetrant, high-frequency, susceptibility genes for lung cancer.

High-Penetrant, Low-Frequency Genes for Lung Cancer

A single gene for lung cancer has not yet been identified, although lung cancer does occur, on occasion, in families with Li-Fraumeni syndrome, a condition that is associated with inherited p53 mutations.10 Large, multigeneration pedigrees with multiple affected family members need to be accrued for genome-wide searches for lung cancer genes. The difficulties in pursuing a single gene for lung cancer are that lung cancer families are rare (occurring in only 1% of the population), onset is usually in the mid to late 60s, and, because of the high case fatality for lung cancer, affected relatives are typically deceased, and smoking data must be collected on all family members, many of whom also may be deceased. These problems necessitate collaborative efforts to identify families for linkage studies. One such collaborative effort is underway by the Genetic Epidemiology of Lung Cancer Consortium.

High-Frequency, Low-Penetrant Genes for Lung Cancer

Genes encoding enzymes that are associated with carcinogen metabolism and DNA repair have been the focus of research into possible susceptibility genes for lung cancer. It has been hypothesized that differences in susceptibility to carcinogens result from an individual’s ability to form genotoxic intermediates, to detoxify these intermediates, and to repair damaged DNA. Polymorphisms in genes coding for the enzymes that drive these processes are likely candidate susceptibility genes.

Some of the most widely and recently studied polymorphic loci coding for phase I and II enzymes involved in the activation and conjugation of tobacco smoke constituents are cytochrome P4501A1 (CYP1A1), glutathione S-transferase M1 (GSTM1), myeloperoxidase (MPO), and NAD(P)H: quione oxidoreductase (NQO1). The results for selected larger studies are presented in Table 1 . The findings of an association between CYP1A1 polymorphisms and a risk of lung cancer have been inconsistent. Studies in Japanese populations1112 have reported risks that were increased over twofold. A meta-analysis13based on 15 studies reported a nonsignificant OR of 1.27 associated with the MspI polymorphism and a nonsignificant OR of 1.62 for the exon 7 polymorphism in CYP1A1. In pooled analyses based on 22 studies, a significant 2.4-fold increase was reported for the homozygote MspI variant, however, this finding was based on very small numbers of cases and controls carrying the risk genotype.14

The gene encoding glutathione S-transferase mu (GSTM1) occurs in the null form in approximately half of the population. One meta-analysis15estimated an overall OR of 1.13 (95% CI, 1.04 to 1.25), suggesting a modest increased risk associated with the GSTM1 null genotype. An updated meta-analysis16 reported similar findings (OR, 1.17; 95% CI, 1.07 to 1.27). A pooled analysis17in whites < 45 years of age also found an OR of 1.1 (95% CI, 0.9 to 1.3), while a study18on an African-American population reported a twofold increased risk (95% CI, 1.07 to 4.11) among those persons with the null genotype. Analyses1921 of combined CYP1A1 and GSTM1 risk genotypes have generally showed risk increases of more than threefold, however, sample sizes have usually been small.

Studies2224 investigating the MPO gene have shown fairly consistent, although not always statistically significant, reductions in the risk of lung cancer to be associated with the variant allele. The largest study conducted,25 however, reported a nonsignificant OR of 1.15. Conflicting findings also have been reported in investigations of a polymorphism in the NQO1 gene, which can act in both carcinogen activation and detoxification. Because of its dual role in metabolism, there is little agreement in the literature about which genotype is the “risk genotype,” making comparisons across studies difficult. Those studies29 that have reported significant associations between NQO1 genotype and lung cancer risk have been based on fairly small sample sizes and/or subgroup analyses. In the largest study,,27 risk was increased at lower levels of cigarette consumption and was decreased at higher levels of cigarette consumption.

Only a few studies have investigated the role of phase I and II enzyme polymorphisms in nonsmoking and early-onset lung cancer cases, populations that may serve to characterize families with an altered metabolism of carcinogens and increased susceptibility. In the author’s Detroit study,4 preliminary analyses of the polymorphisms in CYP1A1, CYP2E1, GSTM1, and NQO1 have been conducted, and no significant risks were noted. A borderline, but nonsignificant, increased risk was seen for early-onset lung cancer in those persons carrying the GSTM1 null allele (OR, 1.4; 95% CI, 1.0 to 2.1). These analyses are ongoing, with planned analyses of gene-environment and gene-gene interactions.

In addition to the investigation of phase I and II enzyme polymorphisms, over the past several years numerous polymorphisms have been identified in DNA repair genes. A review30 of DNA repair polymorphisms and cancer risk detailed study findings. In general, results have not been consistent, with some indication that risk genotypes may be important only where exposures are low (ie, among light smokers and nonsmokers). A full evaluation of this risk associated with these polymorphisms will come as more studies are conducted.

In general, conclusions from studies of candidate susceptibility genes and lung cancer risk have been limited by the low frequency of some polymorphisms in the population, the variability in allele frequencies by ethnicity, alternative definitions of risk genotype, the potential for heterogeneity by histologic type of lung cancer, and the variation in risk associated with the level of exposure to tobacco smoke. To overcome these potential limitations, it will be essential to conduct well-designed, large studies that evaluate risk along a mechanistic pathway that may include several genes, since it is unlikely that one genotype has a strong effect on risk. Even with the difficulties faced in the search for susceptibility genes for lung cancer and the lack of strong findings to date, the role of lung cancer susceptibility genes should not be underestimated. These genes may be of high frequency and, therefore, may be associated with a substantial attributable risk in the population, even when the risk of lung cancer is only moderately increased or decreased.

There is strong evidence suggesting a genetic predisposition for lung cancer. Studies of familial aggregation have shown familial risk on the same order of that reported for breast and colon cancer. More limited research has been done to determine the pattern of inheritance for lung cancer in families, but two studies78 have reported evidence for contributions by a major gene for lung cancer. While a single gene has not yet been identified, efforts are underway to search for a high-penetrant, low-frequency gene for lung cancer. Several low-penetrant, high- frequency genes for lung cancer have been studied, focusing on genes encoding for enzymes involved in the metabolism of carcinogens and DNA repair. These studies have produced somewhat conflicting findings, and, when significant, only modest associations have been reported. Identifying risk genes will be facilitated by improved technology, the conduct of large population-based studies of candidate susceptibility genes, and gene-gene and gene-environment interactions, and linkage studies with genome-wide searches for single genes. Little progress has been made in the early detection and treatment of lung cancer. Given that it is the leading cause of death from cancer, it is exceedingly important to identify high-risk populations and to better understand the carcinogenic process. The identification of individuals with a genetic predisposition will aid in these efforts.

Abbreviations: CI = confidence interval; CYP1A1 = cyto-chrome P4501A1; GSTM1 = glutathione S-transferase M1; MPO = myeloperoxidase; OR = odds ratio

This research was supported in part by National Cancer Institute grants R29-CA50383 and RO1-CA60691, and by contract NO1-CN65064.

Table Graphic Jump Location
Table 1. Selected Association Studies of Candidate Susceptibility Loci for Phase I and Phase II Enzymes
Reis LAG, Eisner MP, Kosary CL, et al, eds. SEER cancer statistics review, 1975–2000: National Cancer Institute. Available at: http://SEER.cancer.gov/csr/1975_2000. Accessed April 12, 2004.
 
Mattson, ME, Pollack, ES, Cullen, JW What are the odds that smoking will kill you?Am J Public Health1987;77,425-431
 
Ooi, WL, Elston, RC, Chen, VW, et al Increased familial risk for lung cancer.J Natl Cancer Inst1986;76,217-222
 
Schwartz, AG, Yang, P, Swanson, GM Familial risk of lung cancer among nonsmokers and their relatives.Am J Epidemiol1996;144,554-562
 
Mayne, ST, Buenconsejo, J, Janerich, DT Familial cancer history and lung cancer risk in United States nonsmoking men and women.Cancer Epidemiol Biomarkers Prev1999;8,1065-1069
 
Bromen, K, Pohlabeln, H, Jahn, I, et al Aggregation of lung cancer in families: results from a population-based case-control study in Germany.Am J Epidemiol2000;152,497-505
 
Sellers, TA, Bailey-Wilson, JE, Elston, RC, et al Evidence for mendelian inheritance in the pathogenesis of lung cancer.J Natl Cancer Inst1990;82,1272-1279
 
Yang, P, Schwartz, AG, McAllister, AE, et al Lung cancer risk in families of nonsmoking probands: heterogeneity by age at diagnosis.Genet Epidemiol1999;17,253-273
 
Yang, P, Schwartz, AG, McAllister, A, et al Genetic analysis of families with nonsmoking lung cancer probands.Genet Epidemiol1997;14,181-197
 
Malkin, D, Li, FP, Strong, LC, et al Germ line p53 mutations in a familial syndrome of breast cancer sarcomas and other neoplasms.Science1990;250,1233-1238
 
Kawajiri, K, Nakachi, K, Imai, K, et al Identification of genetically high risk individuals to lung cancer by DNA polymorphisms of the cytochrome P450IA1 gene.FEBS Lett1990;263,131-133
 
LeMarchand, L, Sivaraman, L, Pierce, L, et al Associations of CYP1A1, GSTM1, and CYP2E1 polymorphisms with lung cancer suggest cell type specificities to tobacco carcinogens.Cancer Res1998;58,4858-4863
 
Houlston, RS CYP1A1 polymorphisms and lung cancer risk: a meta-analysis.Pharmacogenetics2000;10,105-114
 
Vineas, P, Veglia, F, Benhamou, S, et al CYP1A1 T3801C polymorphisms and lung cancer: a pooled analysis of 2,451 cases and 3,358 controls.Int J Cancer2003;104,650-657
 
Houlston, RS Glutathione S-transferase M1 status and lung cancer risk: a meta-analysis.Cancer Epidemiol Biomarkers Prev1999;8,675-682
 
Benhamou, S, Lee, WJ, Alexandrie, A-K, et al Meta- and pooled analysis of the effects of glutathione S-transferase M1 polymorphisms and smoking on lung cancer risk.Carcinogenesis2002;23,1343-1350
 
Tailio, E, Gaspari, L, Benhamou, S, et al Polymorphisms in CYP1A1, GSTM1, GSTT1 and lung cancer below the age of 45 years.Int J Epidemiol2003;32,60-63
 
Ford, JG, Li, Y, O’Sullivan, MM, et al Glutathione S-transferase M1 polymorphism and lung cancer risk in African-Americans.Carcinogenesis2000;21,1971-1975
 
Quinones, L, Lucas, D, Godoy, J, et al CYP1A1, CYP2E1, and GSTM1 genetic polymorphisms: the effect of single and combined genotypes on lung cancer susceptibility in Chilean people.Cancer Lett2001;174,35-44
 
Hayashi, S, Watanabe, J, Kawajiri, K High susceptibility to lung cancer analyzed in terms of combined genotypes of P4501A1 and Mu-class glutathione S-transferase genes.Jpn J Cancer Res1992;83,866-870
 
Alexandrie, A-K, Sundberg, MI, Seidegard, J, et al Genetic susceptibility to lung cancer with special emphasis on CYP1A1 and GSTM1: a study on host factors in relation to age at onset, gender and histological cancer types.Carcinogenesis1994;15,1785-1790
 
Schabath, M, Spitz, M, Hong, WK, et al A myeloperoxidase polymorphism associated with reduced risk of lung cancer.Lung Cancer2002;37,35-40
 
Le Marchand, L, Seifried, A, Lum, A, et al Association of the myeloperoxidase−463G→ A polymorphism with lung cancer risk.Cancer Epidemiol Biomarkers Prev2000;9,181-184
 
Feyler, A, Voho, A, Bouchardy, C, et al Point: myeloperoxidase−463G→ A polymorphism and lung cancer risk.Cancer Epidemiol Biomarkers Prev2002;11,1550-1554
 
Xu, L-L, Liu, G, Miller, DP, et al Counterpoint: the myeloperoxidase−463G→ A polymorphism does not decrease lung cancer susceptibility in Caucasians.Cancer Epidemiol Biomarkers Prev2002;11,1555-1559
 
Wiencke, JK, Spitz, MR, McMillan, A, et al Lung cancer in Mexican-Americans and African-Americans is associated with the wild-type genotype of the NAD(P)H: quinone oxidoreductase polymorphism.Cancer Epidemiol Biomarkers Prev1997;6,87-92
 
Xu, LL, Wain, JC, Miller, DP, et al The NAD(P)H: quinone oxidoreductase 1 gene polymorphism and lung cancer: differential susceptibility based on smoking behavior.Cancer Epidemiol Biomarkers Prev2001;,303-309
 
Sunaga, N, Kohno, T, Yanagitani, N, et al Contribution of NQO1 and GSTT1 polymorphisms to lung adenocarcinoma susceptibility.Cancer Epidemiol Biomarkers Prev2002;11,730-738
 
Chen, H, Lum, A, Seifried, A, et al Association of the NAD(P)H: quinone oxidoreductase 609C→ T polymorphism with a decreased lung cancer risk.Cancer Res1999;59,3045-3048
 
Goode, E, Ulrich, CN, Potter, JD Polymorphisms in DNA repair genes and associations with cancer risk.Cancer Epidemiol Biomarkers Prev2002;11,1513-1530
 

Figures

Tables

Table Graphic Jump Location
Table 1. Selected Association Studies of Candidate Susceptibility Loci for Phase I and Phase II Enzymes

References

Reis LAG, Eisner MP, Kosary CL, et al, eds. SEER cancer statistics review, 1975–2000: National Cancer Institute. Available at: http://SEER.cancer.gov/csr/1975_2000. Accessed April 12, 2004.
 
Mattson, ME, Pollack, ES, Cullen, JW What are the odds that smoking will kill you?Am J Public Health1987;77,425-431
 
Ooi, WL, Elston, RC, Chen, VW, et al Increased familial risk for lung cancer.J Natl Cancer Inst1986;76,217-222
 
Schwartz, AG, Yang, P, Swanson, GM Familial risk of lung cancer among nonsmokers and their relatives.Am J Epidemiol1996;144,554-562
 
Mayne, ST, Buenconsejo, J, Janerich, DT Familial cancer history and lung cancer risk in United States nonsmoking men and women.Cancer Epidemiol Biomarkers Prev1999;8,1065-1069
 
Bromen, K, Pohlabeln, H, Jahn, I, et al Aggregation of lung cancer in families: results from a population-based case-control study in Germany.Am J Epidemiol2000;152,497-505
 
Sellers, TA, Bailey-Wilson, JE, Elston, RC, et al Evidence for mendelian inheritance in the pathogenesis of lung cancer.J Natl Cancer Inst1990;82,1272-1279
 
Yang, P, Schwartz, AG, McAllister, AE, et al Lung cancer risk in families of nonsmoking probands: heterogeneity by age at diagnosis.Genet Epidemiol1999;17,253-273
 
Yang, P, Schwartz, AG, McAllister, A, et al Genetic analysis of families with nonsmoking lung cancer probands.Genet Epidemiol1997;14,181-197
 
Malkin, D, Li, FP, Strong, LC, et al Germ line p53 mutations in a familial syndrome of breast cancer sarcomas and other neoplasms.Science1990;250,1233-1238
 
Kawajiri, K, Nakachi, K, Imai, K, et al Identification of genetically high risk individuals to lung cancer by DNA polymorphisms of the cytochrome P450IA1 gene.FEBS Lett1990;263,131-133
 
LeMarchand, L, Sivaraman, L, Pierce, L, et al Associations of CYP1A1, GSTM1, and CYP2E1 polymorphisms with lung cancer suggest cell type specificities to tobacco carcinogens.Cancer Res1998;58,4858-4863
 
Houlston, RS CYP1A1 polymorphisms and lung cancer risk: a meta-analysis.Pharmacogenetics2000;10,105-114
 
Vineas, P, Veglia, F, Benhamou, S, et al CYP1A1 T3801C polymorphisms and lung cancer: a pooled analysis of 2,451 cases and 3,358 controls.Int J Cancer2003;104,650-657
 
Houlston, RS Glutathione S-transferase M1 status and lung cancer risk: a meta-analysis.Cancer Epidemiol Biomarkers Prev1999;8,675-682
 
Benhamou, S, Lee, WJ, Alexandrie, A-K, et al Meta- and pooled analysis of the effects of glutathione S-transferase M1 polymorphisms and smoking on lung cancer risk.Carcinogenesis2002;23,1343-1350
 
Tailio, E, Gaspari, L, Benhamou, S, et al Polymorphisms in CYP1A1, GSTM1, GSTT1 and lung cancer below the age of 45 years.Int J Epidemiol2003;32,60-63
 
Ford, JG, Li, Y, O’Sullivan, MM, et al Glutathione S-transferase M1 polymorphism and lung cancer risk in African-Americans.Carcinogenesis2000;21,1971-1975
 
Quinones, L, Lucas, D, Godoy, J, et al CYP1A1, CYP2E1, and GSTM1 genetic polymorphisms: the effect of single and combined genotypes on lung cancer susceptibility in Chilean people.Cancer Lett2001;174,35-44
 
Hayashi, S, Watanabe, J, Kawajiri, K High susceptibility to lung cancer analyzed in terms of combined genotypes of P4501A1 and Mu-class glutathione S-transferase genes.Jpn J Cancer Res1992;83,866-870
 
Alexandrie, A-K, Sundberg, MI, Seidegard, J, et al Genetic susceptibility to lung cancer with special emphasis on CYP1A1 and GSTM1: a study on host factors in relation to age at onset, gender and histological cancer types.Carcinogenesis1994;15,1785-1790
 
Schabath, M, Spitz, M, Hong, WK, et al A myeloperoxidase polymorphism associated with reduced risk of lung cancer.Lung Cancer2002;37,35-40
 
Le Marchand, L, Seifried, A, Lum, A, et al Association of the myeloperoxidase−463G→ A polymorphism with lung cancer risk.Cancer Epidemiol Biomarkers Prev2000;9,181-184
 
Feyler, A, Voho, A, Bouchardy, C, et al Point: myeloperoxidase−463G→ A polymorphism and lung cancer risk.Cancer Epidemiol Biomarkers Prev2002;11,1550-1554
 
Xu, L-L, Liu, G, Miller, DP, et al Counterpoint: the myeloperoxidase−463G→ A polymorphism does not decrease lung cancer susceptibility in Caucasians.Cancer Epidemiol Biomarkers Prev2002;11,1555-1559
 
Wiencke, JK, Spitz, MR, McMillan, A, et al Lung cancer in Mexican-Americans and African-Americans is associated with the wild-type genotype of the NAD(P)H: quinone oxidoreductase polymorphism.Cancer Epidemiol Biomarkers Prev1997;6,87-92
 
Xu, LL, Wain, JC, Miller, DP, et al The NAD(P)H: quinone oxidoreductase 1 gene polymorphism and lung cancer: differential susceptibility based on smoking behavior.Cancer Epidemiol Biomarkers Prev2001;,303-309
 
Sunaga, N, Kohno, T, Yanagitani, N, et al Contribution of NQO1 and GSTT1 polymorphisms to lung adenocarcinoma susceptibility.Cancer Epidemiol Biomarkers Prev2002;11,730-738
 
Chen, H, Lum, A, Seifried, A, et al Association of the NAD(P)H: quinone oxidoreductase 609C→ T polymorphism with a decreased lung cancer risk.Cancer Res1999;59,3045-3048
 
Goode, E, Ulrich, CN, Potter, JD Polymorphisms in DNA repair genes and associations with cancer risk.Cancer Epidemiol Biomarkers Prev2002;11,1513-1530
 
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