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Increased Urinary 8-Isoprostaglandin F2α Is Associated With Lower Plasma Selenium Levels and Lower Vegetable and Fruit Intake in an Asbestos-Exposed Cohort at Risk for Lung Cancer* FREE TO VIEW

E. Brigitte Gottschall, MD, MSPH; Pam Wolfe, MS; Albert D. Haegele, BS; Zongjian Zhu, PhD; Cecile S. Rose, MD, MPH; Tricia Sells, BS; Henry J. Thompson, PhD
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*From the National Jewish Medical and Research Center (Drs. Gottschall and Rose and Ms. Sells), Denver, CO; and Colorado State University (Ms. Wolfe, Mr. Haegele, and Drs. Zhu and Thompson), Fort Collins, CO.

Correspondence to: E. Brigitte Gottschall, MD, MSPH, 1400 Jackson St, G215, Denver, CO 80206; e-mail: gottschallb@njc.org

Chest. 2004;125(5_suppl):83S. doi:10.1378/chest.125.5_suppl.83S
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Oxidative damage is a putative mechanism in the pathogenesis of asbestos-related lung diseases, including lung cancer. We previously have shown that the lipid peroxidation product 8-isoprostaglandin F2α (8-EPG) in urine was positively associated with years of asbestos exposure and smoking status in an asbestos-exposed cohort. We analyzed the influence of diet (ie, fruit and vegetable intake) and plasma selenium levels (a marker of selenium status) on urinary 8-EPG levels in this population.

Seventy-nine asbestos-exposed construction trades workers completed a seven-item food frequency questionnaire that was validated for assessing daily fruit and vegetable intake. An administered questionnaire elicited age, smoking status, and asbestos exposure. After solid phase extraction, we measured urinary 8-EPG concentrations using an enzyme-linked immunosorbent assay kit. Plasma selenium levels were determined by a fluorometric procedure.

We found an inverse association between urinary 8-EPG levels, and both daily fruit/vegetable intake and plasma selenium levels. This association remained significant after controlling for age, current smoking status, and duration of asbestos exposure. The regression model showed a strong interaction between smoking status and self-reported daily fruit/vegetable consumption. For 16 current smokers and 63 former smokers or never-smokers, the standardized regression coefficients were markedly different at β̂ = −0.67 (p = 0.01) and, β̂ = −0.23 (p = 0.06), respectively.

The oxidant injury marker urinary 8-EPG is significantly higher in asbestos-exposed workers reporting low fruit/vegetable intake, particularly in smokers. Low plasma selenium levels are also predictors of higher 8-EPG levels. These findings may have implications for preventive interventions such as dietary modification and selenium supplementation in cohorts that are at risk for lung cancer. The association between urinary 8-EPG levels and biomarkers of lung cancer risk in sputum is currently under investigation in this cohort.

Abbreviation: 8-EPG = 8-isoprostaglandin F2α

This research was supported by National Cancer Institute grants K-23 CA84034, R-01 CA84059, and M01-RR00051.




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