0
Laboratory and Animal Investigations |

Oral N-Acetylcysteine Attenuates Elastase-Induced Pulmonary Emphysema in Rats*

Maria L. Rubio; M. Carmen Martin-Mosquero; Mercedes Ortega; German Peces-Barba; Nicolás González-Mangado
Author and Funding Information

*From the Laboratorio Neumología Experimental, Servicio de Neumología, Fundación Jiménez Díaz, Universidad Autónoma, Madrid, Spain.

Correspondence to: Nicolás González Mangado, MD, PhD, Servicio de Neumología, Fundación Jiménez Díaz Avda/ Reyes Católicos, 2 28040-Madrid, Spain; e-mail: ngonzalez@fjd.es



Chest. 2004;125(4):1500-1506. doi:10.1378/chest.125.4.1500
Text Size: A A A
Published online

Study objective: To study the effect of the antioxidant N-acetylcysteine (NAC) in the development of elastase-induced emphysema in rats.

Materials and methods: Wistar rats (n = 72) were orotracheally instilled with 75 IU elastase or saline solution. Eighteen rats from each group received the antioxidant NAC from 2 days before induction of the lesion until they were killed 2, 8, and 28 days after instillation. The effects of treatment were assessed by measuring collagen content for the left lung, a histopathology evaluation (ie, mean alveolar internal surface area (AIA) and mean linear intercept measurement), and lung function.

Results: Twenty-eight days after elastase instillation, rats treated with NAC showed significant attenuation of the lesion in comparison with rats treated only with elastase, including the following: normalization of mean (± SEM) collagen content (1.23 ± 0.09 vs 1.51 ± 0.10 mg per left lung, respectively; p < 0.05); partial inhibition of mean AIA (14,860 ± 1,135 vs 19,622 ± 1,294 μm2, respectively; p < 0.05) and mean linear intercept (108.8 ± 3.7 vs 123.0 ± 4.2 μm, respectively; p < 0.05); and increases and improvement in expiratory flows (27.8 ± 1.2 vs 23.4 ± 1.3 mL/s, respectively; p < 0.05). NAC was not able to avoid the compliance increase in the elastase-plus-NAC group.

Conclusion: Consistent with the results of anatomic, pathologic, and functional studies, NAC is able to attenuate the lesions induced by elastase in rats, which is in accordance with previous data supporting the idea that oxidant injury could contribute to the development of elastase-induced emphysema.

Figures in this Article

Sign In to Access Full Content

MEMBER & INDIVIDUAL SUBSCRIBER

Want Access?

NEW TO CHEST?

Become a CHEST member and receive a FREE subscription as a benefit of membership.

Individuals can purchase this article on ScienceDirect.

Individuals can purchase a subscription to the journal.

Individuals can purchase a subscription to the journal or buy individual articles.

Learn more about membership or Purchase a Full Subscription.

INSTITUTIONAL ACCESS

Institutional access is now available through ScienceDirect and can be purchased at myelsevier.com.

Sign In to Access Full Content

MEMBER & INDIVIDUAL SUBSCRIBER

Want Access?

NEW TO CHEST?

Become a CHEST member and receive a FREE subscription as a benefit of membership.

Individuals can purchase this article on ScienceDirect.

Individuals can purchase a subscription to the journal.

Individuals can purchase a subscription to the journal or buy individual articles.

Learn more about membership or Purchase a Full Subscription.

INSTITUTIONAL ACCESS

Institutional access is now available through ScienceDirect and can be purchased at myelsevier.com.

Figures

Tables

References

NOTE:
Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).

Some tools below are only available to our subscribers or users with an online account.

Sign In to Access Full Content

MEMBER & INDIVIDUAL SUBSCRIBER

Want Access?

NEW TO CHEST?

Become a CHEST member and receive a FREE subscription as a benefit of membership.

Individuals can purchase this article on ScienceDirect.

Individuals can purchase a subscription to the journal.

Individuals can purchase a subscription to the journal or buy individual articles.

Learn more about membership or Purchase a Full Subscription.

INSTITUTIONAL ACCESS

Institutional access is now available through ScienceDirect and can be purchased at myelsevier.com.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Find Similar Articles
CHEST Journal Articles
PubMed Articles
  • CHEST Journal
    Print ISSN: 0012-3692
    Online ISSN: 1931-3543