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Laboratory and Animal Investigations |

T Helper Type 2 Cytokine Receptors and Associated Transcription Factors GATA-3, c-MAF, and Signal Transducer and Activator of Transcription Factor-6 in Induced Sputum of Atopic Asthmatic Patients*

Rame Taha; Qutayba Hamid; Lisa Cameron; Ron Olivenstein
Author and Funding Information

*From the Meakins-Christie Laboratories and Montreal Chest Research Institute (Drs. Taha, Hamid, and Olivenstein), McGill University, Montreal, QC, Canada; and Respiratory Sciences Center (Dr. Cameron), University of Arizona, Tempe, AZ.

Correspondence to: Ron Olivenstein, MD, Montreal Chest Institute, McGill University, 3650 St. Urbain St, Montreal, QC, Canada H2X 2P4; Tel: (514) 849-5201; e-mail: rolive3@po-box.mcgill.ca



Chest. 2003;123(6):2074-2082. doi:10.1378/chest.123.6.2074
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Background: It is well-known that the expression of T helper (Th) type 2 cytokines such as interleukin (IL)-4 and IL-5, and their receptors, is up-regulated within the airways of allergic asthmatic patients. Furthermore, higher numbers of cells producing GATA-3, c-MAF, and signal transducer and activator of transcription factor (STAT)-6, which are transcription factors (TFs) that are implicated in the regulation and signaling of the Th2 cytokines, have been observed in bronchial biopsy specimens from asthmatic patients but not in those of healthy control subjects.

Methods: We examined whether these mediators also can be detected in induced sputum. Immunoreactivity for IL-4Rα, IL-5Rα, GATA-3, c-MAF, and STAT-6 was investigated in samples of induced sputum from asthmatic patients (n = 8) and healthy control subjects (n = 8).

Results: Our results showed that the numbers of cells expressing IL-4 receptor α (Rα) and IL-5Rα were higher in samples from asthmatic patients compared to those of control subjects (p < 0.01). More cells exhibiting GATA-3, c-MAF, and STAT-6 immunoreactivity also were found in asthmatic patients vs those in control subjects (p < 0.005). Furthermore, the expression of STAT-6 and IL-4Rα, GATA-3 and IL-5Rα, and c-MAF with both IL-4Rα and IL-5Rα was correlated (p < 0.05).

Conclusions: This study demonstrated that induced sputum provides sufficient sensitivity for examining the pathways of cytokine signaling, cytokine receptor signaling, and intracellular signaling. Furthermore, these data show correlations between the expression of Th2 cytokine receptors and associated TFs in the human lung, which has not been documented previously.

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