0
Laboratory and Animal Investigations |

Neutrophil Chemotactic Activity of Sputum From Patients With COPD*: Role of Interleukin 8 and Leukotriene B4

Kai M. Beeh; Oliver Kornmann; Roland Buhl; Sarah V. Culpitt; Mark A. Giembycz; Peter J. Barnes
Author and Funding Information

*From Thoracic Medicine (Drs. Beeh, Culpitt, Giembycz, and Barnes), National Heart and Lung Institute, Faculty of Medicine, Imperial College of Science, Technology & Medicine, London, UK; and Pulmonary Department (Drs. Kornmann and Buhl), Internal Medicine, University Hospital, Mainz, Germany.

Correspondence to: Peter J. Barnes, MD, Thoracic Medicine, National Heart and Lung Institute, Faculty of Medicine, Imperial College of Science, Technology & Medicine, London SW7 2AZ, UK; e-mail: p.j.barnes@ic.ac.uk



Chest. 2003;123(4):1240-1247. doi:10.1378/chest.123.4.1240
Text Size: A A A
Published online

Study objectives: Neutrophilic inflammation is a major feature of COPD. Several factors in bronchial secretions have been identified as chemoattractants for neutrophils. The present study was designed to assess the contribution of interleukin (IL)-8 and leukotriene B4 (LTB4) to neutrophil chemotaxis evoked by sputum obtained from patients with established COPD.

Design: Sputum supernatant of 20 patients with COPD was used as chemoattractant in a 96-well chemotaxis chamber, with subsequent quantification of migrated cells by a luminescence assay. The contribution of IL-8 and LTB4 to chemotaxis was determined by addition of a neutralizing antibody and a selective receptor antagonist, respectively.

Measurements and results: COPD sputum caused neutrophil chemotaxis in a concentration-dependent manner, with a maximum response evoked with a 10-fold dilution of the original sample. Pretreatment of sputum or neutrophils with either an anti–IL-8 antibody or the LTB4 antagonist, SB 201146, led to a concentration-dependent inhibition of sputum-induced neutrophil chemotaxis, with a maximum suppression (mean ± SEM) of 29.2 ± 4.9% (p < 0.001) from baseline by 100 ng/mL of anti–IL-8 antibody, and 45.6 ± 7% (p < 0.02) by 10 μmol/L of SB 201146. The combination of the anti–IL-8 antibody and SB 201146 inhibited neutrophil chemotaxis, but this was not significantly greater than the effect of SB 201146 or anti–IL-8 alone.

Conclusions: These data confirm the importance of IL-8 and LTB4 as chemoattractants for neutrophils in bronchial secretions from patients with COPD, and suggest that specific inhibitors may have therapeutic potential in COPD.

Figures in this Article

Sign In to Access Full Content

MEMBER & INDIVIDUAL SUBSCRIBER

Want Access?

NEW TO CHEST?

Become a CHEST member and receive a FREE subscription as a benefit of membership.

Individuals can purchase this article on ScienceDirect.

Individuals can purchase a subscription to the journal.

Individuals can purchase a subscription to the journal or buy individual articles.

Learn more about membership or Purchase a Full Subscription.

INSTITUTIONAL ACCESS

Institutional access is now available through ScienceDirect and can be purchased at myelsevier.com.

Sign In to Access Full Content

MEMBER & INDIVIDUAL SUBSCRIBER

Want Access?

NEW TO CHEST?

Become a CHEST member and receive a FREE subscription as a benefit of membership.

Individuals can purchase this article on ScienceDirect.

Individuals can purchase a subscription to the journal.

Individuals can purchase a subscription to the journal or buy individual articles.

Learn more about membership or Purchase a Full Subscription.

INSTITUTIONAL ACCESS

Institutional access is now available through ScienceDirect and can be purchased at myelsevier.com.

Figures

Tables

References

NOTE:
Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).

Some tools below are only available to our subscribers or users with an online account.

Sign In to Access Full Content

MEMBER & INDIVIDUAL SUBSCRIBER

Want Access?

NEW TO CHEST?

Become a CHEST member and receive a FREE subscription as a benefit of membership.

Individuals can purchase this article on ScienceDirect.

Individuals can purchase a subscription to the journal.

Individuals can purchase a subscription to the journal or buy individual articles.

Learn more about membership or Purchase a Full Subscription.

INSTITUTIONAL ACCESS

Institutional access is now available through ScienceDirect and can be purchased at myelsevier.com.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Find Similar Articles
CHEST Journal Articles
PubMed Articles
  • CHEST Journal
    Print ISSN: 0012-3692
    Online ISSN: 1931-3543