A large number of known as well as unknown differentially expressed
genes were identified. A number of the known genes, such as those
involved in B-cell Ig production (ie, IgG-γ and Ig-ε
heavy chain and terminal deoxynucleotidyltransferase) and in T-cell
activation (ie, signal transducer and activator of
transcription 1, IL-2R, and interferon-γR) confirmed the
validity of this strategy. The complementary DNA fragments thus
obtained have been used to generate custom complementary DNA arrays.
These arrays are hybridized with labeled RNAs from specific tissues or
cell lines, or from the same tissues after different experimental
treatments or at different time points. Genes with interesting patterns
of expression and/or encoding proteins of potential interest for
asthma were selected for further functional studies. The ultimate
challenge is to use this knowledge to explore new therapies for the
treatment of patients with allergic asthma that are aimed at the root
causes of the disease.