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Lipopolysaccharide-Induced Neutrophil Gene Expression Under In Vivo or In Vitro Conditions*

John Arcaroli, MS; John Kupfner, BS; Ho-Kee Yum, MD; Robert Shenkar, PhD; Jong Sung Park, PhD; Edward Abraham, MD
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*From the Division of Pulmonary Sciences and Critical Care Medicine, University of Colorado Health Science Center, Denver, CO.

Correspondence to: John Arcaroli, MS, Division of Pulmonary Sciences and Critical Care Medicine, University of Colorado Health Sciences Center, 4200 E 9th Ave, Box C272, Denver, CO 80262



Chest. 2002;121(3_suppl):76S-77S. doi:10.1378/chest.121.3_suppl.76S
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Extract

Endotoxemia is a frequent precipitating etiology for acute lung injury. Acute lung injury is characterized by the accumulation of activated neutrophils in the lungs. Although neutrophils can be stimulated in vitro by exposure to lipopolysaccharide (LPS), it is likely that in vivo interactions with other pulmonary cell populations modulate the activation patterns in neutrophils migrating to the lungs after endotoxemia.

To study the effects of LPS on neutrophil activation in vivo, lung neutrophils were isolated from male Balb/c mice 1 h after LPS administration (1 mg/kg intraperitoneally). For in vitro studies, bone marrow neutrophils were isolated and stimulated with LPS (100 ng/mL) for 1 h. Mu11KsubA and Mu11KsubB chips were used to determine messenger RNA expression patterns after LPS exposure in vivo and in vitro.

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