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Enhancement of Adenovirus-Mediated Gene Transfer in Lungs and Epithelial Cells by EGTA*

Carpantato Myles, BS; Eric Sorscher, MD; Sadis Matalon, PhD
Author and Funding Information

*From the Department of Anesthesiology, Medicine and Cystic Fibrosis Research Center, University of Alabama at Birmingham, Birmingham, AL.

Correspondence to: Sadis Matalon, PhD, Associate Dean of Postdoctural Education, THT 940, 1900 University Blvd, Birmingham, AL 35233; e-mail: Sadis.Matalon@ccc.uab.edu



Chest. 2002;121(3_suppl):35S. doi:10.1378/chest.121.3_suppl.35S
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We investigated the enhancement of gene transfer across epithelial cells using adenoviral vectors by the addition of low doses of ethylene glycol-bis(β-aminoethylether)-N,N,N′,N′-tetra-acetic acid (EGTA). In the first series of experiments, we instilled intratracheally in CD-1 mice 108 plaque-forming units of adenoviral vector-containing reporter genes with and without 100 μM EGTA. Significant expression of luciferase was found in lung tissue 7 days later; addition of 100 μM EGTA in the instillate increased adenoviral luciferase expression by 600%.

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