investigated the enhancement of gene transfer across epithelial cells
using adenoviral vectors by the addition of low doses of ethylene
(EGTA). In the first series of experiments, we instilled
intratracheally in CD-1 mice 108 plaque-forming
units of adenoviral vector-containing reporter genes with and without
100 μM EGTA. Significant expression of luciferase was found in lung
tissue 7 days later; addition of 100 μM EGTA in the instillate
increased adenoviral luciferase expression by 600%.