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Laboratory and Animal Investigations |

Use of Glass Capillaries Avoids the Time Changes in High Blood Po2 Observed With Plastic Syringes*

Marie Pia d’Ortho, MD, PhD; Christophe Delclaux, MD, PhD; Françoise Zerah, MD; Robert Herigault; Serge Adnot, MD, PhD; Alain Harf, MD, PhD
Author and Funding Information

*From the Service de Physiologie-Explorations Fonctionnelles and Institut National de la Santé et de la Recherche Médicale, INSERM U492, Hôpital Henri Mondor, Hôpitaux de Paris, Créteil, France.

Correspondence to: Alain Harf, MD, PhD, Service de Physiologie-Explorations Fonctionnelles, Hôpital Henri Mondor, 94010 Créteil, France; e-mail: alain.harf@hmn.ap-hop-paris.fr



Chest. 2001;120(5):1651-1654. doi:10.1378/chest.120.5.1651
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Study objectives: In adults, arterial blood samples are usually drawn using plastic syringes. In contrast to glass syringes, plastic syringes let oxygen diffuse through their wall. This results in Po2 changes during storage, especially when Po2 is high. An alternative to glass syringes is the Microsampler (Roche Diagnostics; Schaffhausen, Switzerland), a commercially available device consisting of a heparinized glass capillary fitted with a 26-gauge needle and used to collect arterial blood in the same way as a plastic syringe fitted with a needle.

Design: We evaluated the performance of the Roche Microsampler for storing arterial blood in view of Po2 measurement, comparatively with glass and plastic syringes. Five approximate initial Po2 levels (650, 400, 200, 130, and 80 mm Hg) and two storage temperatures (ambient temperature and 4°C) were studied.

Settings: Bench study.

Results: Plastic syringes allowed reliable measurement of Po2 values when initial Po2 was too low to ensure complete hemoglobin oxygen saturation, but were associated with time-dependent underestimation of Po2 at higher initial Po2 values. No such underestimation occurred with the Roche Microsampler stored at 4°C for up to 1 h for all Po2 levels studied.

Conclusion: The Roche Microsamplers appeared to be reliable devices in preventing oxygen diffusion.

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