Study objectives: To assess the frequencies of three
polymorphisms at amino acid positions 16, 27, and 164 of theβ
2-adrenoceptor (β2-AR) gene and their
effects on COPD patients.
Patients: Sixty-five patients with
COPD and 41 healthy subjects were included.
Measurements: Polymorphisms of the β2-AR
coding block were delineated using an allele-specific polymerase chain
reaction (PCR) approach. The allele-specific PCR technique was verified
by direct dideoxy sequencing of PCR products. Pulmonary function tests
were performed in all patients.
Results: The Arg16β
2-AR polymorphism was less prevalent in COPD patients
than in healthy populations (p = 0.01). A significant correlation
(p < 0.018) between the Gln27 β2-AR polymorphism and
FEV1 percent predicted value was found. Patients
with the Gln27 polymorphism had a higher percentage of low
FEV1 percent predicted than did patients with the GlnGlu
and GluGlu variants.
Conclusions: The polymorphism of
Gly16 may increase the patient’s susceptibility to the development of
COPD. The Gln27 β2-AR polymorphism may be associated with
the severity of COPD in a Chinese