Objectives: We tested the hypothesis that an increase
in the blood colloid osmotic pressure (COP) that is maintained during
early-stage endotoxemia may decrease fluid flux across capillaries and
may reduce pulmonary and multiple-organ edema.
Settings: Research laboratory in a
Subjects: Male albino Sprague-Dawley
Interventions: Rats were anesthetized with
pentobarbital, underwent tracheotomies, were cannulated in the femoral
vein and artery, and were randomly assigned to the following four
groups comprising 11 rats each: group I, controls (saline solution
treatment); group II, albumin treatment (three doses of 1 g/kg 25%
human albumin every 2 h); group III, endotoxin treatment with a
single IV dose of 4 mg/kg endotoxin; and group IV, endotoxin and
albumin-treatment (4 mg/kg endotoxin plus albumin treatment).
Experiments lasted for 6 h while fluid intake was equally
maintained in all groups.
Measurements and results:
COP and other variables were measured every 2 h. To determine the
water content of an organ, after the rat was killed, the lung, heart,
kidney, intestine, and liver were removed. Albumin treatment alone
(group II) generated significant increases in COP (maximum, 58% from
the baseline measurement) but did not change the water content of the
organ, compared with saline solution-treated controls.
Endotoxin-treated rats (group III) developed significant reductions in
COP, with significant increases in pulmonary, renal, and heart water
content compared with controls. Albumin treatment in endotoxemic rats
(group IV) significantly increased the COP without improving the
endotoxemia-induced organ edema. Pulmonary edema, however, was
increased further, compared with endotoxemia alone.
Conclusions: COP elevation by albumin administration during
the early stage of endotoxemia does not ameliorate pulmonary or
multiple-organ edema and may aggravate pulmonary