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Laboratory and Animal Investigations |

High Concentrations of β-Chemokines in BAL Fluid of Patients With Diffuse Panbronchiolitis*

Jun-ichi Kadota, MD; Hiroshi Mukae, MD; Kazunori Tomono, MD; Shigeru Kohno, MD, FCCP
Author and Funding Information

Affiliations: *From the Second Department of Internal Medicine, Nagasaki University School of Medicine, Nagasaki, Japan. ,  Currently at Oita Medical University.

Correspondence to: Jun-ichi Kadota, MD, Second Department of Internal Medicine, Oita Medical University, 1-1 Hasama-machi, Oita 879-5593, Japan; e-mail: kadota@oita-med.ac.jp



Chest. 2001;120(2):602-607. doi:10.1378/chest.120.2.602
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Background: T cells are important cellular components of bronchial inflammation in diffuse panbronchiolitis (DPB).β -Chemokines such as RANTES (regulated on activation, normal T-cell expressed and secreted) and macrophage inflammatory peptide (MIP)-1α are closely related to the migration of inflammatory cells into the lung. In this study, we investigate the contribution of β-chemokines to the accumulation of T cells in the lungs of patients with DPB.

Patients and methods: We determined the levels ofβ -chemokines in BAL fluid (BALF) and the correlation between these levels and T-cell subsets in BALF of 23 patients with DPB and 16 healthy subjects by sandwich enzyme-linked immunosorbent assay and flow cytometry.

Results: Percentages of CD3+ human leukocyte antigen (HLA)-DR+, CD8+, and CD8+HLA-DR+ cells in BALF of patients were significantly higher than in the control BALF. The absolute number of CD8+HLA-DR+ cells was also higher in BALF of patients than in the control BALF (p < 0.0001). Phenotypic analysis of CD4+ cells in BALF showed a similar percentage of CD4+CD45RA+ cells and CD4+CD29+ cells in patients and normal subjects. The concentrations of RANTES and MIP-1α in BALF of patients with DPB were significantly higher than in BALF of normal subjects (p < 0.05). In addition, there was a significant correlation between the absolute number or percentage of CD8+HLA-DR+ cells and MIP-1α concentration in BALF.

Conclusions: Our results suggest that the interaction between activated CD8+ T cells and MIP-1α may contribute to the pathogenesis of DPB.

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