Objective: The fluorescence of tissue when stimulated
by a laser beam is a well-known phenomenon. The resulting emission
spectra depend on the biochemical and structural composition of the
tissue. In this study, we examined the spectra of laser-induced
fluorescence emitted by myocardial tissue.
used an argon-ion laser to stimulate the myocardium of 20 intact sheep
hearts. For each spectral emission, we calculated the intensity in
specific regions in order to characterize the spectra and to reveal
intercavitary and intracavitary morphologic differences.
Results: The statistical analysis showed significant
differences in the emission spectra intensity between atria and
ventricles. The intensity was higher in the atria than in the
ventricles (p < 0.001). The atrial emission spectra were
morphologically different from those of the ventricles. There was no
difference in the intensity or morphology of emission spectra within
each chamber. All measurements showed good reproducibility after a
short period of time.
fluorescence of myocardial tissue seems to have the characteristics
necessary for tissue recognition. This might prove useful in
identifying cardiomyopathies and transplant rejection, as well as for
myocardial mapping, assisting electrophysiologists in discovering
fibrotic arrhythmogenic foci.