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Laboratory and Animal Investigations |

Elevated Levels of Soluble Adhesion Molecules in Sera and BAL Fluid of Individuals Infected With Human T-Cell Lymphotropic Virus Type 1*

Masafumi Seki, MD; Yasuhito Higashiyama, MD, PhD; Jun-ichi Kadota, MD, PhD; Hiroshi Mukae, MD, PhD; Katsunori Yanagihara, MD, PhD; Kazunori Tomono, MD, PhD; Shigeru Kohno, MD, FCCP
Author and Funding Information

*From the Second Department of Internal Medicine, Nagasaki University School of Medicine, Nagasaki, Japan.

Correspondence to: Shigeru Kohno, MD, FCCP, Second Department of Internal Medicine, Nagasaki University School of Medicine, Sakamoto 1–7-1, Nagasaki 852-8501, Japan; e-mail:s-kohno@net.nagasaki-u.ac.jp



Chest. 2000;118(6):1754-1761. doi:10.1378/chest.118.6.1754
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Study objective: T-lymphocytic alveolitis and increased levels of interleukin-2 receptor-α (CD25)-bearing T cells in the BAL fluid (BALF) of human T-cell lymphotropic virus type 1 (HTLV-1) carriers have been reported. Several chemokines and adhesion molecules may contribute to the accumulation of T lymphocytes in the lungs of HTLV-1 carriers. To clarify the correlation between adhesion molecules and HTLV-1-associated pulmonary disorders, we compared the distribution of T-lymphocyte subsets and soluble adhesion molecules, including soluble intercellular adhesion molecule (sICAM)-1, soluble vascular cell adhesion molecule-1 (sVCAM-1), soluble L-selectin (sL-selectin), soluble E-selectin (sE-selectin), and soluble P-selectin (sP-selectin), in BALF and peripheral blood, between HTLV-1 carriers and noninfected healthy subjects.

Design: Flow cytometric analysis with monoclonal antibodies to cell-surface antigens was used to identify T-lymphocyte subsets in BALF samples from HTLV-1 carriers (n = 13) and noninfected healthy control subjects (n = 10). The levels of various soluble adhesion molecules in serum and in BALF were estimated by enzyme-linked immunosorbent assay.

Results: Higher percentages of CD3+ cells, CD3-expressing human leukocyte antigen-DR antigen, and CD3+CD25+ cells were detected in the BALF of HTLV-1 carriers than in that of noninfected control subjects. The concentrations of sICAM-1, sVCAM-1, sL-selectin, SE- selectin, and sP-selectin in the sera of patients were significantly higher than those in noninfected healthy control subjects. The concentration of sICAM-1 in the BALF of patients was significantly higher than that in noninfected healthy control subjects, and the concentration of sICAM-1 correlated well with the percentage of CD3+CD25+ cells.

Conclusion: The concentrations of adhesion molecules in the sera of and sICAM-1 in the BALF of HTLV-1 carriers were significantly higher than those in noninfected individuals, and the concentration of sICAM-1 correlated well with the percentage of CD3+CD25+ cells in BALF. Our results suggest a possible interaction between activated T cells bearing CD25 and soluble adhesion molecules, especially sICAM-1, which may contribute to the pulmonary involvement in HTLV-1 carriers.

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