Study objectives: The etiology of sarcoidosis remains
unknown, but recently it was reported that human herpesvirus 8 (HHV-8)
may be detected in sarcoid tissue in a high proportion of patients.
This study was performed to determine whether HHV-8 is implicated in
sarcoidosis in Japan.
Materials and methods:
Peripheral blood mononuclear cells (PBMCs) were obtained from 100
patients with sarcoidosis and 100 healthy donors living in central
Japan. Additionally, 19 samples of sarcoid tissue, 10 of tuberculous
tissue, and 10 of lung cancers were examined. DNA was extracted from
PBMC or tissue samples, and a hemi-nested polymerase chain reaction
assay was performed for HHV-8 detection.
the PBMC study, the detection rates for HHV-8 in patients with
sarcoidosis and in normal donors were 2% and 1%, respectively, the
difference not being significant (p > 0.99). In the tissue study,
HHV-8 was detected in 10.5% of sarcoid and in 15% of nonsarcoid
tissues, again not a significant difference (p > 0.99).
Conclusions: From these results, we conclude that HHV-8 is
not implicated in sarcoidosis in Japanese patients.