0
Clinical Investigations: COPD |

Tumor Necrosis Factor Gene Complex in COPD and Disseminated Bronchiectasis*

Cristina Patuzzo, BiSci; Lucia S. Gilè, MD; Michele Zorzetto, BiSci; Elisabetta Trabetti, BiSci; Giovanni Malerba, BiSci; Pier Franco Pignatti, MD, PhD; Maurizio Luisetti, MD, FCCP
Author and Funding Information

*From the Istituto di Biologia e Genetica, Università degli Studi di Verona (Professor Pignatti, Drs. Patuzzo, Trabetti, and Malerba); Laboratorio di Biochimica e Genetica, Clinica di Malattie dell’Apparato Respiratorio, IRCCS Policlinico San Matteo, Università degli Studi di Pavia (Drs. Luisetti and Zorzetto); Fondazione S. Maugeri, Gussago (BS) (Dr. Gilè), Italy.

Correspondence to: Maurizio Luisetti, MD, FCCP, Laboratorio di Biochimica e Genetica, Clinica di Malattie dell’Apparato Respiratorio, IRCCS Policlinico San Matteo, Italy; e-mail: maurizio.luisetti@tin.it



Chest. 2000;117(5):1353-1358. doi:10.1378/chest.117.5.1353
Text Size: A A A
Published online

Background: Tumor necrosis factor (TNF) is a potent proinflammatory cytokine with increased levels in the sputum of COPD subjects. Two biallelic TNF gene complex polymorphisms have been described: LtαNcoI, in the first intron of the lymphotoxin α (previously referred to as TNF-β) gene, and TNF-308, in the promoter region of the TNF-α gene. Higher levels of TNF production are associated with allele 1 of LtαNcoI (LtαNcoI*1) and with allele 2 of TNF-308 (TNF-308*2).

Study objectives: To study the frequencies of the two TNF gene complex polymorphisms in patients with COPD and bronchiectasis.

Design: Association study.

Subjects and methods: We studied the frequencies of these polymorphisms in 66 subjects with COPD and in 23 subjects with disseminated bronchiectasis and compared them to the frequencies in 98 healthy control subjects and 45 subjects with nonobstructive pulmonary disease. Genomic DNA samples were extracted, and TNF-α and LtαNcoI polymorphisms were detected after polymerase chain reaction by restriction digestion.

Results: We found the following frequencies: the TNF-308*2 allele was detected in 11% of COPD individuals, 15% of bronchiectasis patients, 10% of healthy control subjects, and 18% of subjects with nonobstructive pulmonary disease. The LtαNcoI*1 allele was detected in 28% of COPD individuals, 30% of bronchiectasis patients, 29% of healthy control subjects, and 29% of subjects with nonobstructive pulmonary disease. We found evidence of linkage disequilibrium between the two loci (Δ = 0.068).

Conclusions: We conclude that the TNF gene complex, at least in Caucasoid individuals and for the considered polymorphisms, does not seem to play a major role as genetic risk factor in COPD and bronchiectasis.

Figures in this Article

Sign In to Access Full Content

MEMBER & INDIVIDUAL SUBSCRIBER

Want Access?

NEW TO CHEST?

Become a CHEST member and receive a FREE subscription as a benefit of membership.

Individuals can purchase this article on ScienceDirect.

Individuals can purchase a subscription to the journal.

Individuals can purchase a subscription to the journal or buy individual articles.

Learn more about membership or Purchase a Full Subscription.

INSTITUTIONAL ACCESS

Institutional access is now available through ScienceDirect and can be purchased at myelsevier.com.

Sign In to Access Full Content

MEMBER & INDIVIDUAL SUBSCRIBER

Want Access?

NEW TO CHEST?

Become a CHEST member and receive a FREE subscription as a benefit of membership.

Individuals can purchase this article on ScienceDirect.

Individuals can purchase a subscription to the journal.

Individuals can purchase a subscription to the journal or buy individual articles.

Learn more about membership or Purchase a Full Subscription.

INSTITUTIONAL ACCESS

Institutional access is now available through ScienceDirect and can be purchased at myelsevier.com.

Figures

Tables

References

NOTE:
Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).

Some tools below are only available to our subscribers or users with an online account.

Sign In to Access Full Content

MEMBER & INDIVIDUAL SUBSCRIBER

Want Access?

NEW TO CHEST?

Become a CHEST member and receive a FREE subscription as a benefit of membership.

Individuals can purchase this article on ScienceDirect.

Individuals can purchase a subscription to the journal.

Individuals can purchase a subscription to the journal or buy individual articles.

Learn more about membership or Purchase a Full Subscription.

INSTITUTIONAL ACCESS

Institutional access is now available through ScienceDirect and can be purchased at myelsevier.com.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Find Similar Articles
CHEST Journal Articles
PubMed Articles
  • CHEST Journal
    Print ISSN: 0012-3692
    Online ISSN: 1931-3543