Study objectives: To investigate whether oxidative
stress occurs following lobectomy and pneumonectomy and to evaluate
whether markers of oxidative stress might be of value in the assessment
of the diagnosis, course, and prognosis of postoperative
Design: A prospective study.
Setting: A specialized thoracic surgical unit in a large
Patients: Twenty-eight patients
with lung carcinoma undergoing thoracotomy.
Measurements: Exhaled H2O2
concentrations in breath condensate were measured by spectrophotometry,
while malondialdehyde (MDA) levels in urine samples collected every 24
h were measured by reversed-phase, ion-pair high-performance
liquid chromatography using ultraviolet detection.
Results: Our results show increased
H2O2 and MDA levels in lobectomy patients
compared with pneumonectomy patients. A strong correlation was found
between the levels of H2O2 and MDA.
Conclusion: The present data support the hypothesis
that oxidative stress may occur following pulmonary