0
Clinical Investigations: LUNG CANCER |

B-Cell Pulmonary Lymphoma*: Gene Rearrangement Analysis of Bronchoalveolar Lymphocytes by Polymerase Chain Reaction

Bruno Philippe, MD; Marie-Hélène Delfau-Larue, MD; Bernard Epardeau, MD; Brigitte Autran, MD; Jean-Pierre Clauvel, MD; Jean-Pierre Farcet, MD; Louis-Jean Couderc, MD
Author and Funding Information

*From the Departments of Pulmonary Diseases (Drs. Philippe and Couderc) and Pathology (Dr. Epardeau), Foch Hospital, Suresnes; the Laboratory of Biological Immunology (Drs. Delfau-Larue and Farcet), Henri Mondor Hospital, Créteil; the Laboratory of Cellular Immunology (Dr. Autran), Pitié-Salpétrière Hospital, Paris; and the Department of Clinical Immunology and Hematology (Dr. Clauvel), Saint-Louis Hospital, Paris, France.

Correspondence to: Bruno Philippe, MD, Hôpital Foch, Service de Pneumologie, 40 rue Worth, BP 36, F-92151 Suresnes Cedex, France; e-mail: bphil@pasteur.fr



Chest. 1999;115(5):1242-1247. doi:10.1378/chest.115.5.1242
Text Size: A A A
Published online

Study objectives: Non-Hodgkin’s lymphomas (NHLs) are clonal proliferation of B or T lymphocytes. Assessment of clonality in lymphoid proliferations uses immunochemistry and, recently, molecular biology. The aim of our study is to assess the role of immunoglobulin gene rearrangement analysis on bronchoalveolar lymphocytes to aid in the diagnosis of B-cell pulmonary NHL.

Patients and methods: The study took place in a university hospital. There were seven consecutive patients with B-cell-type pulmonary lymphoma and nine control subjects. Gene rearrangement analysis using polymerase chain reaction (PCR) technique was performed on alveolar lymphocytes recovered by BAL.

Results: Analysis of the immunoglobulin heavy chain gene rearrangement showed a predominant clonal alveolar lymphocyte population in six of seven patients while all control subjects showed germline pattern.

Conclusions: Gene rearrangement analysis by PCR of alveolar lymphocytes would appear to be sensitive in patients with B-cell pulmonary NHL (six of seven patients) and specific (zero of nine in the control group). This simple test should be added only in the analysis of cells recovered by BAL in patients with suspected primary and secondary B-cell pulmonary NHL.

Abbreviations: NHL = non-Hodgkin’s lymphomas; PCR = polymerase chain reaction; TCR = T-cell receptor

Figures in this Article

Sign In to Access Full Content

MEMBER & INDIVIDUAL SUBSCRIBER

Want Access?

NEW TO CHEST?

Become a CHEST member and receive a FREE subscription as a benefit of membership.

Individuals can purchase this article on ScienceDirect.

Individuals can purchase a subscription to the journal.

Individuals can purchase a subscription to the journal or buy individual articles.

Learn more about membership or Purchase a Full Subscription.

INSTITUTIONAL ACCESS

Institutional access is now available through ScienceDirect and can be purchased at myelsevier.com.

Sign In to Access Full Content

MEMBER & INDIVIDUAL SUBSCRIBER

Want Access?

NEW TO CHEST?

Become a CHEST member and receive a FREE subscription as a benefit of membership.

Individuals can purchase this article on ScienceDirect.

Individuals can purchase a subscription to the journal.

Individuals can purchase a subscription to the journal or buy individual articles.

Learn more about membership or Purchase a Full Subscription.

INSTITUTIONAL ACCESS

Institutional access is now available through ScienceDirect and can be purchased at myelsevier.com.

Figures

Tables

References

NOTE:
Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).

Some tools below are only available to our subscribers or users with an online account.

Sign In to Access Full Content

MEMBER & INDIVIDUAL SUBSCRIBER

Want Access?

NEW TO CHEST?

Become a CHEST member and receive a FREE subscription as a benefit of membership.

Individuals can purchase this article on ScienceDirect.

Individuals can purchase a subscription to the journal.

Individuals can purchase a subscription to the journal or buy individual articles.

Learn more about membership or Purchase a Full Subscription.

INSTITUTIONAL ACCESS

Institutional access is now available through ScienceDirect and can be purchased at myelsevier.com.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Find Similar Articles
CHEST Journal Articles
PubMed Articles
  • CHEST Journal
    Print ISSN: 0012-3692
    Online ISSN: 1931-3543