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Anti-Kp 90 IgA Antibodies in the Diagnosis of Active Tuberculosis

Sevtap Arikan; Serdar Tuncer; Dürdal Us; Serhat Ünal; Şemsettin Ustaçelebi
Author and Funding Information

From the Hacettepe University School of Medicine, Ankara, Turkey

Dürdal Us, PhD, Hacettepe University School of Medicine, Department of Clinical Microbiology and Microbiology, 06100 Ankara, Turkey


1998 by the American College of Chest Physicians


Chest. 1998;114(5):1253-1257. doi:10.1378/chest.114.5.1253
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Abstract

Background: Accurate diagnosis of active tuberculosis (TB) has been difficult historically, yet a great demand persists for a rapid and reliable diagnostic method. Detection of Mycobacterium tuberculosis anti-Kp 90 IgA antibodies is one of the more novel techniques.

Study objectives: To evaluate the diagnostic value of a recently developed enzyme-linked immunosorbent assay (ELISA) test, which detects IgA antibodies against M tuberculosis Kp 90 antigen, and to compare the results with conventional diagnosis and the polymerase chain reaction (PCR) method.

Participants: Serum, ethylenediaminetetraacetic acid (EDTA)-blood, and body fluid samples were obtained from 51 patients with active TB and 71 control subjects. The clinical diagnosis of TB was supported by a positive culture (n = 6), detection of acid-fast bacilli on smear (n = 35), or both (n = 10).

Measurements and results: IgA antibodies were detected in sera and/or body fluid samples from 82% of patients with TB and 10% of controls. M tuberculosis DNA was detected in body fluid sample of 96% and blood sample of 49% of patients with TB by PCR. None of the blood and 5.6% of the body fluid specimens from controls were PCR-positive.

Conclusions: Anti-Kp 90 IgA antibodies were detected using ELISA in 78% of serum and 69% of body fluids from patients with TB, therefore, this test is promising for the diagnosis of active TB and appears to be more reliable, particularly for body fluid samples.


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