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BAL Fluid Contains Detectable Superoxide Dismutase 1 Activity

Robert A. DiSilvestro; Eric Pacht; W. Bruce Davis; Nizar Jarjour; Hyojee Joung; Kathleen Trela-Fulop
Author and Funding Information

Affiliations: From Human Nutrition and Food Management, The Ohio State University, Columbus,  From the Department of Medicine, Division of Pulmonary and Critical Care, The Ohio State University Medical Center, Columbus,  From the Division of Pulmonary and Critical Care Medicine, University of Wisconsin Medical School, Madison

Affiliations: From Human Nutrition and Food Management, The Ohio State University, Columbus,  From the Department of Medicine, Division of Pulmonary and Critical Care, The Ohio State University Medical Center, Columbus,  From the Division of Pulmonary and Critical Care Medicine, University of Wisconsin Medical School, Madison

Affiliations: From Human Nutrition and Food Management, The Ohio State University, Columbus,  From the Department of Medicine, Division of Pulmonary and Critical Care, The Ohio State University Medical Center, Columbus,  From the Division of Pulmonary and Critical Care Medicine, University of Wisconsin Medical School, Madison


1998 by the American College of Chest Physicians


Chest. 1998;113(2):401-404. doi:10.1378/chest.113.2.401
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Published online

Abstract

Objective: This study determined which, if any, of the three superoxide dismutase (SOD) enzyme activities were detectable in BAL fluid (BALF).

Background: The identity and concentrations of antioxidant molecules in BALF have not been fully characterized. One important class of antioxidants is that of the SOD enzymes.

Methods: BALF from control nonsmokers (n=9), smokers (n=7), and asthmatic subjects (n=12) were examined for SOD activity by a modified pyrogallol auto-oxidation method. The particular SOD enzyme or enzymes responsible for any activity were identified based on activity inhibition data and gel filtration column chromatography patterns.

Results: SOD activity was detected in all samples. Unlike serum or some other fluids, in which the enzyme extracellular SOD accounts for virtually all SOD activity, the enzyme SOD 1 accounted for virtually all SOD activity. BALF SOD activities were lower for smokers than for control or asthmatic subjects (p<0.05).

Conclusion: BALF SOD 1 activities can be measured as part of lung antioxidant studies. Data from a limited number of subjects suggested that smokers can have low BALF SOD values.


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