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Alveolar Fluid Glutathione Decreases in Asymptomatic HIV-Seropositive Subjects Over Time

Eric R. Pacht; Phil Diaz; Tom Clanton; Judy Hart; James E. Gadek
Author and Funding Information

From the Division of Pulmonary and Critical Care, Department of Internal Medicine, The Ohio State University Medical Center, Columbus


1997 by the American College of Chest Physicians


Chest. 1997;112(3):785-788. doi:10.1378/chest.112.3.785
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Abstract

Background: Initial investigations demonstrated a deficiency of glutathione (GSH) in the epithelial lining fluid (ELF) of HIV-seropositive patients. In a recent study, our laboratory was unable to document such a deficiency. The current study was performed in an attempt to reconcile those disparate findings.

Study objectives: To determine if ELF GSH decreases over time in asymptomatic HIV-seropositive subjects.

Design: Prospective, longitudinal study.

Setting: Major university medical center.

Patients or participants: Thirty-three asymptomatic HIV-seropositive volunteers.

Interventions: None.

Measurements and results: BAL was performed on 33 asymptomatic HIV-seropositive subjects at baseline, 6 months later, and 12 months later. The volume of ELF and the concentration of GSH and oxidized GSH were determined. The concentration of total GSH in ELF was 689.0±100.4 µM. This significantly decreased when measured 6 and 12 months later (355.9±41.7 µM, and 397.9±52.7 µM, respectively, p=0.01, compared with baseline, both comparisons). Significant decreases were also noted in the HIV-seropositive subjects who smoked cigarettes (baseline—762.6±142.4 µM; 6 months—373.7±45.9 µM; 12 months—459.3±73.8 µM, p<0.03, for baseline vs 6 months, and baseline vs 12 months). In nonsmoking HIV-seropositive subjects, there was a decrease in ELF GSH over time, but it did not reach statistical significance (baseline—589.1±138.2 µM; 6 months—335.3±74.1 µM; 12 months—345.8±74.0 µM, p>0.1, all comparisons). The percentage of total GSH in the oxidized form was similar at all three time points (baseline—3.8±0.5%; 6 months—3.1±0.5%; 12 months—3.9±0.9%, p>0.1, all comparisons).

Conclusions: The current study demonstrates that the GSH level in ELF is significantly decreased in HIV-seropositive subjects 6 and 12 months after the initial determination.


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