PURPOSE: A possible genetic predisposition to tuberculosis has been suggested in several studies, but the functional immunologic correlate of the genetic polymorphisms identified is often unclear. Up to date, previous studies reported that some gene single nucleotide polymorphism at Korean increase susceptibility to pulmonary tuberculosis, but the study of vitamin D receptor polymorphism was not reported. We are expecting that this study will show a immunegenetics of tuberculosis infection in Korean
METHODS: This study was designed as prospective, not-interventional, observational method. New patient with tuberculosis infection and heath control will be enrolled according to the inclusion and exclusion criteria. All researcher will get informed consent from all participants Sample Collection: DNA will be extracted from blood samples using the Puregene DNA Isolation kit (Gentra Systems, Minneapolis, MN) following the manufacturer’s protocol. SNP Genotyping: Pyrosequencing SNP genotyping assays from Pyomark system. SNP genotyping will be done according to manufacturer’s manual. Target gene Vitamin D Fok1 A/G rs10735810 TaqI rs731236 BsmI rs1544410 Sample size: healthy control 150 Person, TB control 150 Person
RESULTS: Normal control 156 persons and TB control 160 persons were enrolled. Mean age of normal control was 44.22±17.7 control was 47.22±19. Sex (male: female) was 84:72 in normal control and 89:71 in TB control. Pulmonary TB(98 person: 61%) and Extrapulmonary TB(62 person:38%) was enrolled. In BsmI, CC (94.1%), TC(5.2%), TT(0.3%) for normal control and CC(92.1%) TC(8.3%) TT(0.5%) for TB control were detected. In TaqI, AA(88.2%) GA(11.6%) GG(0.2%) for normal control, AA(89.8%) GA(9.8%) GG(0.5%) for TB control were detected. In Fok1, CC(29.4%), TC(47.1%) TT(23.5%) for normal control and CC(29.8%), TC(54.1%) TT(16.1%) for TB control. In FokI, the frequency of TT minor allele was 23.5% in normal control and 15.1% in TB group(odd 0.65 CI: 0.22-1.88, P=0.495) and 18.3% in pulmonary TB and 12.7% in extrapulmonary TB group(odd 0.859 CI: 0.66-1.1, P=0.333). In Bsm and TaqI, there was no significant data to show the influence of minor allele.
CONCLUSIONS: Vitamin D receptor polymorphism did not influenced the susceptibility of tuberculosis among south Korean.
CLINICAL IMPLICATIONS: Vitamin D receptor polymorphism may affect limited susceptiblity of tuberculosis among south korean.
DISCLOSURE: The following authors have nothing to disclose: Jusang Kim, Joong Hyun Ahn, Chan Kwon Park, HyoungKyu Yoon, Ji Young Kang, Hyeon Hui Kang, Sang Haak Lee, Ki Hoon Park
No Product/Research Disclosure Information