PURPOSE: Malignant pleural mesothelioma(MPM) is increasing in the world, and treatment resistant and a poor prognosis are observed. The poor prognosis is due to the aggressive local invasiveness rather than distant metastasis. In this study, the relationship between the expression of MT1-MMP and the invasion ability of cell lines was investigated using the simple method that we created previously.
METHODS: We used the clinical specimen which we got from the patient with malignant mesothelioma, and established cell lines from them. We employed an easy-to-prepare double-layered collagen gel hemisphere (DL-CGH) system that demonstrated the advantages for visualizing cell movements during invasion and observed the invasive activities of MPM cells. To assess the role of MT1-MMP in invasive activity of MPM cells, knock-down of the expression by RNA interference (RNAi) was performed.
RESULTS: Invasion assay with DL-CGH revealed that high expression of MT1-MMP in MPM cells showed aggressive invasive activity. RNAi of MT1-MMP indicated that Expression of MT1-MMP might have crucial role in invasiveness of MPM cells.
CONCLUSIONS: MT1-MMP expression in MPM cells related to the ability of locally aggressive spreading into the pleura and the surrounding tissues, and MT1-MMP will be a molecular target to suppress the invasion of MPM.
CLINICAL IMPLICATIONS: MT1-MMP expression in MPM cells suggests the ability of locally aggressive spreading into the pleura and the surrounding tissues, and MT1-MMP will be a molecular target to suppress the invasion of MPM.
DISCLOSURE: The following authors have nothing to disclose: Masahiro Yoshimura, Yoshimasa Maniwa, Wataru Nishio
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