PURPOSE: Fibroblasts, macrophages and mast cells orchestrated by T cells participate in fibrotic processes in humans. They are major source of Oncostatin M cytokine of the IL-6 family that stimulates proliferation of fibroblasts and production of collagen. Sarcoidosis, in less then 15% of patients it going to chronic process with devastating fibrosing changes in the lung. The human mast cells express of Oncostatin M on activation by T cells. Our assumption was that increased levels of mast cells expressing Oncostatin M in the bronchoalveolar lavage (BAL) of patients with sarcoidosis would correlate with continuing chronic inflammation and fibrosis - pulmonary function tests.
METHODS: Twelve Sarcoidosis patients between 2008- 2010 were eligible for the study and underwent bronchoscopy. The diagnosis was done according to the ATS guideline. Local Ethics Committee approved the study. All patients underwent pulmonary function test. BAL was performed before biopsies. Cytospins slides from BAL were prepared. Methylene blue staining performed for total cell count. Samples incubated with the primary antibody rabbit anti-human c-kit, CD117 for overnight at 40C and stained for total mast cell count. The second primary antibody mouse anti-human Oncostatin M applied and activated mast cells count were made.
RESULTS: Mean FEV1/FVC was 78.38% with range (52-87) and STD-9.3%. Avarage of total mast cell count per slide was 179.56 and activated mast cells-stained with Oncostatin M was 1.53. Correlation was noted between FEV1/FVC and numbers of acivated mast cells. (Pearson r-0.578. Sig. .049).
CONCLUSIONS: Mast cells are a possible source of OSM in this T cell mediated disease
CLINICAL IMPLICATIONS: This study can help in the future more undestand the patogenesis of sarcoidosis
DISCLOSURE: The following authors have nothing to disclose: Alexander Guber, Jawad Abdelqader, Pazit Salamon, Yosef Mekori, David Shitrit
No Product/Research Disclosure Information