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Poster Presentations: Tuesday, October 25, 2011 |

The Influence of Regulatory T Cells on Early Differentiation of Th Cells in Asthmatic Patients FREE TO VIEW

Jing Liu, PhD; Ke Wang, PhD; Jie Zhang, PhD; Zhongsen Ma, MD
Chest. 2011;140(4_MeetingAbstracts):222A. doi:10.1378/chest.1115493
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Abstract

PURPOSE: To study the the influence of Treg cells on early differentiation of Th0 cells to Th1,Th2,Th17 and Th9 cells in asthmatic patients.

METHODS: Recruited asthmatic patients (n=20) and healthy control (n=20), and isolated CD4+CD25+CD127-/low Treg cells and CD4+CD25-CD62L+ Th0 cells with immunomagnetic beads method (purity was above 90%). In different incentive condition, cultured Th0 cells to differentiate to Th1, Th2, Th17 and Th9 cells with or without Treg cells. After cultured for 5 days, measured the expression of T-bet, GATA3, RORr and IL-9 in different cultured cells with RT-PCR method, and the production of IFNr, IL-13, IL-17 and IL-9 in culture supernatant with ELISA methods.

RESULTS: Treg cells could suppress the proliferation of Th0 cells in both asthmatic and healthy groups, but the suppressive capacity of Treg cells in asthmatic groups was lower than that in healthy group(P<0.05). In vitro, Th0 cells could differentiate to Th1, Th2, Th17 and Th9 cells respectively in different incentive condition, with specific gene express of T-bet, GATA3, RORr and IL-9 and cytokines production of IFNr, IL-13, IL-17 and IL-9. Treg cells suppressed the differentiation of Th0 cells to Th1, Th2 and Th17 cells in both asthmatic and healthy groups (P<0.05). The suppressive capacity of Treg cells on differentiation of Th0 cells to Th17 and Th2 cells in asthmatic groups was less effective than in healthy group(P<0.05).

CONCLUSIONS: There was deficiency for the suppress capacity of Treg cells on differentiation of Th0 cells to Th17 and Th2 cells in asthmatic groups.

CLINICAL IMPLICATIONS: These data support that the function deficiency of Treg cells on early differentiation of Th cells may be the key point in the development of asthma.

DISCLOSURE: The following authors have nothing to disclose: Jing Liu, Ke Wang, Jie Zhang, Zhongsen Ma

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