PURPOSE: The purpose of this study was to investigate the association between total antioxidant status (FRAP) and markers of oxidative stress (MDA, SOD, GSH) with pulmonary function in patients of Chronic Obstructive Pulmonary Disease.
METHODS: The objective of the present study was to investigate the levels of lipid peroxidation products (Malondialdehyde, MDA), superoxide dismutase (SOD), glutathione and total antioxidant status in normal human volunteers (NHV) and Obstructive Airway Diseased Patients. 1. Total Antioxidant Power (FRAP): The Total antioxidant power in the sample will be carried out by measuring the Ferric reducing ability (Benzie and Strain, 1996). 2. Estimation of Lipid Peroxidation Products (Malondialdehyde) 3. Superoxide dismutase (SOD) activity in erythrocytes 4. Glutathione in blood
RESULTS: : After adjustment for covariates, multiple linear regression analysis showed that total antioxidant status (FRAP) 160.07±63.88 µmol/ml were positively associated with FEV1% and FVC% (P < 0.05, all associations). Erythrocytic glutathione (28.96±6.02 µmol/ml) and SOD 1974.22±503.84 Units were negatively associated with FEV1%, while plasma thiobarbituric acid-reactive substances (TBARS) 2.03±0.44 nmol/ml was negatively associated with FVC% (P < 0.05).
CONCLUSIONS: These results support the hypothesis that an imbalance in antioxidant/oxidant status is associated with chronic airflow limitation, and that oxidative stress play contributing role in severity of the disease.
CLINICAL IMPLICATIONS: Drug therapies directed in correcting these imbalance between various biomarkers (as above) will come up in future.
DISCLOSURE: The following authors have nothing to disclose: Mohammad Shameem, Asrar Ahmad, Qayyum Hussain, Rakesh Bhargava, Zuber Ahmad, Nazish Fatima
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