Several clinical variables were related to colonization, including antipseudomonal antibiotic use (eg, tobramycin and azithromycin, but not colomycin). We suspect that such findings were because of significant numbers of patients receiving tobramycin as opposed to colomycin based on literature that emerged during the study that dictated practice.20,21 All other significant associations suggested that the more advanced a patient’s CF, the more likely that the sputum contained C albicans, a statement corroborated by other researchers.8,22 Following multivariate analysis, however, several of these associations were nullified, and the strongest factors predicting C albicans colonization were pancreatic insufficiency, osteopenia, and cocolonization with Pseudomonas spp. All represent markers of advanced CF and previously have been unidentified in this setting. Prior work has shown increased colonization with long-term antibiotics, CFRD, and prolonged glucocorticoid use.8,22 From our data, antibiotic use and CFRD showed an association with colonization, but neither was among the strongest predictors. One may speculate, however, that although cocolonization with Pseudomonas spp was deemed one of the strongest predictors, this may be attributed in part to the antibiotic treatment used against this organism rather than the organism itself. Our study did not assess corticosteroid use because we infrequently prescribe these agents in CF and, consequently, are unlikely to have adequate numbers to determine whether a true relationship exists.